For the study, Ms Girard collected 120 spiders from the wild and allowed them to court in a natural environment within the laboratory.
The team filmed the courtship using high-definition cameras and used a laser vibrometer to measure the vibrations created by the male spider.
Dr Kasumovic said during 64 mating trials with virgin females, only 16 males were successful.
In 22 trials with mated females, none of the females re-mated.
He said the success by the males was dependent on "how vigorously a male dances and how much attention he pays to his mate".
The fact the mated females would not re-mate suggested they became more selective, Dr Kasumovic said.
His advice to the male peacock spider: "Try your best the first time, as once a female is mated, she gets pickier."
Dr Kasumovic said the female - which will attack and kill the male if she is not happy - did give warnings that the male should try harder.
This involved her wiggling or waving her abdomen, which resulted in the male either backing off or trying harder.
This was not the only time the male spider appeared to be responding to the female's cues, Dr Kasumovic said.
If it appeared she was not paying attention the male would vibrate more and when she would look at him, the male would wave his coloured abdomen more.
"There is a lot of variance and a lot of work involved [in the courtship]. He is really listening to her and changing the courtship and his responses," Dr Kasumovic said.