Smith and her colleagues Bhart-Anjan Bhullar, Gunther Köhler, and Jörg Habersetzer suspect that lampreys evolved their four eyes simultaneously, but they say that more fossil finds will be needed to determine when each of the organs emerged.
What is known is that the earliest mammals — our distant ancestors — did not just have two eyes.
"Stem mammals had three eyes," Smith explained. "It has been proposed that the nocturnal phase of evolution in mammalian ancestors led to the disappearance of the third eye in that lineage, and perhaps something similar happened in other tetrapod groups."
Many tetrapods, or four-footed animals, therefore might have lost their third eye when some became active at night and presumably didn't need the additional photosensory organs.
Humans and most other mammals retain no lingering evidence of a third eye.
"In particular in humans, the cerebrum is so massively expanded that it thickly and completely covers the pineal organ, making it virtually impossible for any photons to reach it," Smith said. "Also, the parapineal organ is regarded as completely absent."
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In rats, however, the pineal organ is close to the surface. Some photosensitive proteins are still expressed in the organ in embryonic rats, suggesting that the third eye didn't completely disappear in the rodent lineage.
Smith and her team are not sure which organ — the pineal or parapineal — led to the formation of the third eye in early mammals. When that mystery is solved, they should gain a better understanding of why the extra eye was lost over time in mammals.
What is clearer is that the third eye of today's lizards, the pineal organ, evolved independently from the third eye of other jawed vertebrates.
"We should stress that the idea that the lizard third eye is not the same as the third eye of frogs or fish was proposed before," Smith said. "But it has not been universally accepted. By finding a species in which both pineal organs were simultaneously developed as eyes, where the front one was clearly the typical lizard third eye, we could confirm this idea."
Although the extinct monitor's extra eyes were located close together, the researchers do not think that they functioned as a pair in the same way that typical eyes do.
"They are indeed closely spaced, but their central neural connections with the rest of the brain are different," Smith explained.
Saniwa ensidens lived in parts of Wyoming and Europe during the Eocene epoch around 48 million years ago. It holds the distinction of being the first fossil lizard ever known from North America. Now it has yet another claim to animal fame — at least for now.
The new finding "tells us how easy it is, in terms of evolution, to self-assemble a complex organ under certain circumstances,” said Bhullar, a paleontologist at Yale. “Eyes are classically thought of as these remarkably complex structures. In fact, the brain is just waiting to make eyes at all times.”
He added that eyes are "fundamentally a part of the brain" and that their formation "is part of the process of how the brain comes in contact with part of the skin."
Smith said that as an embryo develops, the central nervous system begins as an "infolding of the outermost layer of the embryo." This layer is called the neuroectoderm.
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Prior research even determined that octopuses can "see" with their skin. It contains a light-sensitive pigment, rhodopsin, found in eyes. A more recent study found that the color-changing hogfish can also see with its skin. It is possible that other animals, including humans, have skin with light-detecting abilities too.
For now, the researchers are more focused on studying lizards. Smith and Bhullar explained that the so-called "lizard shift" — when lizards first evolved their third eye — is tied to the development of eyesight in a number of different vertebrates. The scientists are hoping to find out when this important change happened.
Smith said that she and her colleagues are looking forward to analyzing the Grizzly Buttes fossil collection again, even though the remains "are quite scrappy and really banged up." She concluded that such investigations "highlight the crucial role played by museums as archives of biodiversity, both past and present."