"None of them, as of now, have shown any traces of nicotine or other alkaloids," the researchers wrote in the journal Rapid Communications in Mass Spectrometry.
Indeed, several issues, such as bacteria, contamination and the fact that the usage of containers changed over time, often limit the success of chemical analysis on ancient residues.
Nevertheless, Loughmiller-Newman and Zagorevski were able to find the chemical fingerprint of tobacco in the codex-style flask.
The identification was performed by using two analytical chemistry techniques - gas chromatography mass spectrometry and liquid chromatography mass spectrometry.
"Both methods resulted in the positive identification of nicotine," said the researchers.
In addition, three oxidation products of nicotine, indicating natural processes of bacterial degradation, were discovered.
BLOG: 2012 Mayan Calendar 'Doomsday' Date Might Be Wrong
None of the nicotine by-products associated with the smoking of tobacco was detected, likely ruling out the use of the vessel as an ashtray.