Wikicommons, Associated Press
In Greek mythology, chimeras were vicious monsters feared by many. This fire-breathing animal had the head and body of a lioness, with a goat head protruding from her back and the tail of a snake. Today, “chimera” refers to an animal that has two or more different sets of genetically distinct cells working together. Remember the mouse with the ear on its back? The movie "Splice" showcases a chimera experiment gone horribly wrong: scientists create a human-animal hybrid that becomes evil and goes completely out of control. While the movie is obviously science fiction, chimera experiments with human cells are not, and real life scientists have been conducting them for decades. We take a look at a few that have been successful in the past and how they’re advancing medicine.
Journal of Cell Research
Rabbit Eggs with Human Cells
The first successful human-animal chimeras were reported in 2003. Chinese researchers at the Shanghai Second Medical University successfully fused human cells with rabbit eggs. They were allowed to develop the eggs for several days in a petri dish before the embryos were harvested for their stem cells. Their hope was that this process could one day be used to grow cells or tissues for transplantation.
University of Minnestoa Mayo Clinic, PBS, Ass
Pigs with Human Blood
A year after the successful Chinese chimera experiment, researchers at the Mayo Clinic in Minnesota announced they had created pigs with human blood pumping through their veins. What was startling about the animal is not only did the pig blood cells flow with human cells, but some of the cells merged together, creating pig-human cell hybrids. Scientists said this experiment can give them a better understanding of how viral infections can pass from animals to humans such as HIV and various others.
University of Nevada-Reno, National Institute
Sheep with Human Livers
One of the efforts behind creating chimeras is to generate animal specimens that could grow human organs to be farmed for transplantation. In 2007, scientists at the University of Nevada-Reno announced they could grow livers made up of 20 percent human cells in sheep. Dr. Esmail Zanjani injected either human adult stem cells derived from bone marrow, or human embryonic stem cells, into growing sheep fetuses. Zanjani said he uses sheep because the circulation systems of sheep and humans are similar.
Salk Institute for Biological Studies
How do you develop treatments for liver infections and diseases only humans can get? Salk Institute researchers came up with one solution in February 2010. Using a mouse that was having liver problems of its own, the researchers replaced its liver with one that was made up of 95 percent human cells to study treatments for Hepatitis. Shown here is a cluster of mouse liver cells that have been replaced with human cells (shown in green). Typically, small animals can't be infected with Hepatitis, only humans and chimps can, but this "humanized" mouse not only became infected, it successfully responded to drug treatments. Scientists believe this experiment could open doors to finding cures for other human liver infections such as malaria.
University of Minnesota, Newcastle University
Cow Eggs with Human Cells
British researchers were given approval to conduct human-animal hybrid research in 2008, a decision that researchers touted would give them the ability to possibly find a cure for Parkinson’s disease. Before, only human cells were allowed to be injected into human eggs, but the researchers argued that animal eggs are much more available. After given permission, researchers went to work using cow eggs. The nucleus of the cow egg -- the source of most DNA and shown here in blue -- was removed, and replaced with the nucleus of a human cell such as a skin cell. Once the egg was allowed to develop and multiply it would become a early-stage cloned embryo called a blastocyst. Scientists could then extract the stem cells from this blastocyst to use in disease treatments.
University of California, Getty Images
Cat-Human Hybrid Proteins
Allergic to cats? Then you’ll appreciate this experiment. The feline Fel d 1 protein found in cat saliva contains an allergen that affects humans. When cats lick themselves, the saliva on their fur dries and turns into dust. In April 2005, scientists at the University of California created a human-cat hybrid when they fused the Fel d 1 protein with a human protein known to suppress allergic reactions. The feline protein would bind to immune cells that would cause the reaction and the human protein would tell the immune cells to calm down. When tested in mice, the chimeric protein stifled the allergy, and researchers hope they can be used in the future to treat allergy sufferers.
Irving Weissman, Stanford University professor and cofounder of the biotech company StemCells Inc., was granted permission by Stanford to create a mouse-human hybrid in 2005. Weissman and his team transplanted human-brain stem cells into the brains of mice with the intention to study neurodegenerative diseases such as Parkinson's and Alzheimer's. In his initial experiment, the human cells only made up less than 1 percent of the mouse brain. Shown here is an isolated mouse brain cell. In 2010, Stanford researchers announced they transformed mouse skin cells into fully functional neurons in a laboratory dish for the first time. They also announced in May that they successfully used mouse stem cells to develop sensory hair cells, which could combat human hearing loss.
George Washington University, DCI
We share over 98 percent of our DNA with chimpanzees, so would it be possible to create a human-chimp hybrid: a "humanzee," also called a "chuman" or "chumanzee"? In the 1920s, a Soviet biologist Ilia Ivanov artificially inseminated female chimps with human sperm, but the pregnancies didn't take. A chimp named Oliver became famous in the 1970s after it was thought he could be a human-chimp hybrid, because he walked upright. However, genetic testing in the 90s proved he was a chimp. Several researchers and citizens see such experiments has highly immoral and there is no known evidence of a human-chimp hybrid.
Ever since Dolly the Sheep was cloned in 1996, scientists have been trying to do the same thing with human cells. Using the same technique, scientists say they've finally accomplished the feat with adult cells.
"What we show for the first time is that you can actually take skin cells, from a middle-aged 35-year-old male, but also from an elderly, 75-year-old male" and use the DNA to create tissue with cells of an exact match, said co-author of the study Robert Lanza.
The work was published in the journal Cell Stem Cell.
Last year, the technique was successfully used with infant cells, but in order to create tissue in a lab that could treat adult diseases, such as Alzheimer's, scientists needed to know if the technique would work with adult cells.
"I'm happy to hear that our experiment was verified and shown to be genuine," said Shoukhrat Mitalipov, a development biologist at Oregon Health and Science University, who led the 2013 study.
The work confirmed that starting with a quality human egg is key to the process. The researchers replaced the original DNA in an unfertilized egg with the donor DNA, and then cultured the cells in a lab dish. The stem cells, which were an exact match to the donor's DNA, can then be turned into various tissue types.
Even though full human cloning is a long way off, the report may raise an equal amount of concern and excitement.
"Certainly this kind of technology could be abused by some kind of rogue scientist," Paul Knoepfler of the University of California, Davis, School of Medicine, told NPR.